Subject(s)
Humans , Monoclonal Gammopathy of Undetermined Significance/diagnosis , Paraproteinemias/classification , Amyloidosis/diagnosis , beta 2-Microglobulin , Heavy Chain Disease/etiology , Immunoelectrophoresis , Immunoglobulins , Interleukin-6/physiology , Waldenstrom Macroglobulinemia/etiology , Multiple Myeloma/etiology , Neoplasm Staging , Paraproteinemias/immunologyABSTRACT
The aim of this work was to assess cellular immunity using the Multitest CMI and relate its results with lymphocyte counts and lymphocyte subpopulations determined using monoclonal antibodies against CD4 and CD8 and fluorescence microscopy. We studied 51 patients (31 males), 20 infected with HIV, 18 with recurring infections, 5 with cancer, 2 with tuberculosis and 6 with miscellaneous diagnoses. According to Multitest results, patients were classified as normal, hypoergic or anergic. Twenty five percent of patients were normal, 65 percent hypoergic and 10 percent anergic. Eighty percent of anergic patients were infected with HIV. No differences in total lymphocyte count were observed between the 3 groups. CD4 lymphocyte count was lower in anergic patients when compared with the other groups. All patients with CD4 counts below 200 cells/mmü were anergic. It is cincluded that Multitest CMI is useful for the assessment of cellular immunity and complements the determination of lymphocyte subpopulations
Subject(s)
Humans , Male , Female , Adult , Lymphocyte Subsets , Lymphocyte Count , Immunity, Cellular/physiology , CD4-Positive T-Lymphocytes , HIV Infections/immunology , Immunologic Techniques/statistics & numerical dataABSTRACT
Delayed hypersensitivity skin test are widely accepted as in vivo measure of cellular immunity. Since genetic and environmental factors may affect these tests, each population must establish its own normal values. The aim of this work was to study delayed hypersensitivity in chilean normal young adults. We studied the response to 8 antigens using the Multitest CMI (Rhodia Merieux) in 50 students (22 females, 28 males), aged 18 to 25 years old. Skin tests were read at 24, 48 and 72 hours. At 48 h, 60 percent of women responded to 3 and 68 percent of men to 4 antigens. A mean response to 3.6 antigens was observed. Total score in men and women were 19.5ñ7.3 and 12.2ñ6.5 mm respectively (p<0.05). These figures are different from these published abroad and confirm the need for national standards
Subject(s)
Humans , Male , Female , Adult , Hypersensitivity, Delayed/diagnosis , Immunity, Cellular/physiology , Skin Tests/methods , Reference Values , Antigens, Bacterial/immunologySubject(s)
Humans , Male , Female , Adult , Arthritis, Rheumatoid/blood , Cryoglobulins/isolation & purification , Cryoglobulinemia/diagnosisABSTRACT
Un niño de 9 años con deficiencia selectiva de inmunoglobulina A (IgA) que fue pesquisado al realizar un estudio sobre valores normales de inmunoglobulinas séricas en niños chilenos sanos del área metropolitana de salud Occidente. Las concentraciones séricas de IgA de este paciente fueron de 0 mg/dl en muestras repetidas. No se encontraron antecedentes familiares, evidencia de enfermedad, ni alteraciones de laboratorio asociadas